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1+ months

Molecular Biologist (Laboratory Management) - NIH - Bethesda, MD

Kelly Services
Bethesda, MD 20814
  • Jobs Rated
    30th
Kelly Government Solutions is a strategic supplier and business partner to the federal government and its key suppliers. Through our partnership with the National Institutes of Health, Kelly Government Solutions offers administrative, IT, engineering, scientific, and healthcare professionals the opportunity to work with the leading medical research center in the world. We are seeking an experienced individual to work as a Laboratory Manager at the National Institutes of Health in Bethesda, MD.


This is a long-term contract position which offers:

- Competitive compensation and comprehensive benefit package


+ Optional health, vision, and dental plans


-Vacation leave as well as 10 paid federal holidays and 401K plan.

- Access to NIHs unparalleled resources and niche scientific initiatives


TASKS/SERVICES. The contractor shall:


**Provides technical experience needed to assist in studying the basic principles of plant and animal life**


+ Plan and administer biological research programs

+ Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function


+ Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species


+ Develop and maintain liaisons and effective working relations with groups and individuals, agencies, and the public to encourage cooperative management strategies or to develop information and interpret findings


+ Collect, analyze and interpret data for all experiments performed


+ Search, retrieve and analyze publicly available data within the cBIOPortal for Cancer Genomics, COSMIC, dbSNP, dbVar, ClinVar, ExAC, The Human Protein Atlas, En-sembl, NCBI databases, DepMap, CCLE, GDSC, DGIdb, UniProtKBSwiss-Prot, and other online databases related to cancer, genomics, and proteomics


+ Make detailed and comprehensive records daily in laboratory notebook, to document: experimental purpose, results and interpretation thereof, and conclusions Laboratory notebook must adhere to NIH policies and lab-specific policies and will be reviewed on a regular basis by the principal investigator


+ Participate in and present research updates at weekly laboratory meetings, and on-on-one meetings with the Principal Investigator, for group and individual discussion


+ Adhere to all institutional policies, safety standards, and completion of appropriate training courses.


+ Perform PubMed and PubMed Central searches and other online searches to identify, read and assimilate scientific and biomedical literature relevant to projects; maintain a personal database of literature in EndNote.


+ Annotate gene and/or protein datasets using Ingenuity Pathway Analysis, g:Profiler, and/or DAVID.


+ Perform hierarchical clustering using Partek Genomics Suite.


+ Perform and/or assist in highly accurate analysis of large-scale datasets.


+ Scan Western blots and perform densitometry, data quantification, and normalization


+ Develop X-ray films after exposure to Western blots.


+ Perform Bradford assays, Western blotting, immunoprecipitation, and co-immunoprecipitation.


+ Perform sequence alignments and sequence format conversions using bioinformatics tools including Clustal Omega, BLAST, and EMBOSS Seqret.


+ Perform macrodissection of fresh-frozen and/or paraffin-embedded human tissues and subsequent protein and nucleic acid extraction, purification, and quantification.


+ Perform protein and nucleic acid isolation and purification from mammalian cell cultures.


+ Perform molecular-based and cell-based assays including migration assays, viability assays, proliferation assays, wound-healing assays, kinase assays, reporter assays, drug sen-sitivity assays, siRNA/shRNA depletion, and in vitro ubiquitination assays.


+ Perform transient and stable transfections of plasmid DNAs into mammalian cell cultures.


+ Use BMG Omega plate reader and Bio-Rad Chemidoc Imager.


+ Perform cell counts using hemocytometer and automated cell counters including the Countess Automated Cell Counter.


+ Perform mammalian cell culture using sterile techniques; freeze stocks of mammalian cells for long-term storage and thaw stocks of frozen mammalian cells to initiate cultures.


+ Perform large-scale bacterial culture, bacterial transformation, bacterial colony growth and selection on agar plates, plasmid DNA isolation, plasmid DNA purification, and plasmid DNA quantification, and gel electrophoresis.


+ Design and generate mammalian expression constructs by site directed mutagenesis and cloning/subcloning.


+ Perform in silico predictions of sequence-variant function, using tools that include Poly-Phen-2, SIFT, Mutation Assessor, PROVEAN, SNAP2, PANTHER-PSEP, and Mut-Pred2.


+ Perform Sanger sequencing, sequence analysis, variant calling, and variant annotation in accordance with Human Genome Variation Society (HGVS) nomenclature.


+ Design and perform polymerase chain reaction (PCR) based assays and perform gel electrophoresis of PCR products.


+ Perform all research and related tasks in a meticulous manner, with scientific integrity, and including appropriate experimental controls, and following pre-approved experimental pro-tocol(s).


+ Perform tasks in a team-setting and/or individual setting, depending on project-specific needs.


**Assists with performing basic laboratory experiments and procedures**


+ Collect and analyze biological data about relationships among and between organisms and their environment


+ Supervise biological technicians and technologists and other scientists


+ Monitor and observe experiments, recording production and test data for evaluation by research personnel


+ Analyze experimental data and interpret results to write reports and summaries of findings


+ Perform all research and related tasks in a meticulous manner, with scientific integrity, and including appropriate experimental controls, and following pre-approved experi-mental protocols


+ Perform tasks in a team-setting andor individual setting, depending on project-specific needs


+ Perform PubMed and PubMed Central searches and other online searches to identify, read and assimilate scientific and biomedical literature relevant to projects; maintain a personal database of literature in EndNote


+ Ship, store, and dispose of biological and hazardous materials according to NIH guide-lines and policies


+ Assist with Western blotting


+ Assist with data analysis


+ Assist with manuscript preparation including preparation of data, tables, and figures.


+ Assist with basic laboratory experiments and procedures


**Prepares, organizes and maintains various lab samples, supplies, and equipment**


+ Operate, calibrate, or maintain equipment used in quantitative or qualitative analysis, such as spectrophotometers, calorimeters, flame photometers, or computer-controlled analyzers


+ Maintain laboratory equipment


+ Operate laboratory equipment to analyze samples


+ Analyze laboratory findings


+ Perform Sanger sequencing, sequence analysis, variant calling, and variant annotation in accordance with Human Genome Variation Society HGVS nomenclature


+ Perform in silico predictions of sequence-variant function using tools that include Poly-Phen-2, SIFT, Mutation Assessor, PROVEAN, SNAP2, PANTHER-PSEP, and Mut-Pred2


+ Perform large-scale bacterial culture, bacterial transformation, bacterial colony growth and selection on agar plates, plasmid DNA isolation, plasmid DNA purification, and plasmid DNA quantification, and gel electrophoresis


+ Perform mammalian cell culture using sterile techniques and freeze stocks of mammalian cells for long-term storage


+ Maintain and organize liquid nitrogen and freezer inventories


+ Perform cell counts using hemocytometer and automated cell counters including the Countess Automated Cell Counter


+ Perform transient and stable transfections of plasmid DNAs into mammalian cell cultures


+ Perform molecular-based and cell-based assays including migration assays, viability as-says, proliferation assays, wound-healing assays, kinase assays, siRNAshRNA depletion, and in vitro ubiquitination assays


+ Perform protein and nucleic acid isolation and purification from mammalian cell cul-tures


+ Perform microdissection of fresh-frozen andor paraffin-embedded human tissues and subsequent protein and nucleic acid extraction, purification, and quantification


+ Perform sequence alignments and sequence format conversions using bioinformatics tools including Clustal Omega, BLAST, and EMBOSS Seqret


+ Perform Bradford assays, Western blotting and immunoprecipitation


+ Perform andor assist in highly accurate analysis of large-scale datasets


+ Perform hierarchical clustering using Partek Genomics Suite


+ Annotate gene andor protein datasets using Ingenuity Pathway Analysis, g: Profiler, andor DAVID


+ Ensures lab complies with green lab program initiatives; may also help complete the green lab assessment if other point-of-contact not available.


+ Establish and maintain laboratory protocols, including SOPs.


+ Communicate in a timely, effective, and civil manner with NHGRI staff, service providers, and other internal and external personnel/stakeholders.


+ Monitor freezers daily for proper temperature; schedule service and/or repair of freezers that are out of normal temperature range; relocate contents of freezer to another location if freezer temperature is out of range


+ Attend and participate in seminars presented by postdoctoral trainees interviewing for positions in the laboratory.


+ Perform freezer defrosts and organization


+ Participate in monthly laboratory manager meetings; disseminate relevant information back to the laboratory for discussion and/or implementation.


+ Respond to laboratory equipment alarms; responses include responding in person to move freezer contents, notifying other lab members of alarm, coordinating responses between lab members during and after an alarm, working to schedule repair of alarming equipment


+ Assist in maintaining internal laboratory databases.


+ Monitor preventative maintenance contracts for equipment and schedule service calls and repairs and act as point of contact with service person when this person is on-site


+ Assist in maintaining internal laboratory biobank.


+ Maintain equipment, ensuring maintenance agreements are current and appropriate


+ Participate in monthly laboratory manager meetings; disseminate relevant information back to the laboratory for discussion and/or implementation.


+ Perform weekly flushes of eye-wash stations at sink(s) and enter into log


+ Keep first-aid box fully stocked with in-date items.


+ Prepare desk space, bench space, on-boarding documents, and prepare order documentation for supplies for new lab members in advance of on-boarding.


+ Assist in management and update of lab-owned property in the property management portal; identify misassigned property and facilitate assignment corrections.


+ Submit facilities management maintenance request tickets and coordinate repair responses.


+ Facilitate the surplus of outdated or excess property.


+ Assess levels of reagents and supplies within the main laboratory and storage areas and communicate with other lab members regarding their upcoming needs; obtain quotes, and process new orders and replacement orders from external vendors to maintain appropriate inventories of critical reagents and supplies.


+ Obtain supplies from the NIH supply store.


+ Participate in management of inventory of critical reagents and supplies


+ Assist with preparation and tracking of documentation for procurement of cell lines from international sources.


+ Assist with preparation and tracking of documentation for procurement of orders through the NIH Consolidated Operations Acquisition Center (COAC).


+ Maintain an updated contact list for Rees alarms for equipment failures including points of contact for individual laboratory equipment as well as shared equipment used by all mem-bers of the Branch.


+ Assist in preparations for participation in annual freezer challenge competition


+ Serve as the primary point of contact for the chemical waste program in the laboratory; schedule chemical waste pick-up; ensure compliance with updated NIH waste disposal guidelines.


+ Assist with participation in the annual NIH Green Labs Program and help ensure compliance with green practices year-round


+ Communicate requirements for the NIH Division of Environmental Protection (DEP) annual lab chemical waste management inspection to lab members.


+ Schedule, prepare for, and facilitate the Division of Occupational Health and Safety (DOHS) annual laboratory survey.


+ Maintain safety training records for the laboratory; notify personnel of upcoming training renewal.


+ Organize and coordinate lab coat laundry service.


+ Receive and properly store delivered supplies up-on delivery; record receiving documents, in order to maintain appropriate inventories while following institutional procurement policies.


+ Ship, store, and dispose of biological and hazardous materials according to institutional guidelines and policies.


**Learns new laboratory techniques and procedures**


+ Program and use computers to store, process, and analyze data


+ Monitor operational procedures in technical environments to ensure conformance to standards


+ Interpret research or operational data


+ Record research or operational data


+ Design and perform polymerase chain reaction PCR based assays and perform gel electrophoresis of PCR products


+ Develop and maintain liaisons and effective working relations with groups and individuals, agencies, and the public to encourage cooperative management strategies or to develop information and interpret findings


+ Design and generate mammalian expression constructs by site directed mutagenesis and cloning subcloning


+ Use BMG Omega plate reader and Bio-Rad Chemidoc Imager


+ Develop X-ray films after exposure to Western blots


+ Scan Western blots and perform densitometry, data quantification, and normalization


+ Learn new institutional policies, safety standards, and complete appropriate training modules


+ Perform weekly flushes of eye-wash stations at sinks and enter into log


+ Keep first-aid box fully stocked with in-date items


+ Learn and communicate requirements for the NIH Division of Environmental Protection DEP annual lab chemical waste management inspection to lab members


+ Learns how to operate new equipment as needed, to meet existing and evolving project goals.


+ Schedule, prepare for, and facilitate the Division of Occupational Health and Safety DOHS annual laboratory survey


+ Monitor safety training records for the laboratory; notify personnel of upcoming training renewal


+ Learns new laboratory techniques and procedures as needed, to meet existing and evolving project goals.


+ Learns new software as needed, to meet existing and evolving project goals.


REQUIREMENTS. The contractor must have:


Certifications & Licenses


+ Three (3) years of specialized experience plus a BA/BS degree is equivalent to a masters degree.

+ Minimum of three (3) years of experience in a science related field.

+ Masters degree in a scientific related field.


Skills (Ranked By Priority)


1. Excellent analytical, organizational and time management skills

2. Strong communications skills, both oral and written.


Software


+ Ability to use Adobe Photoshop and Adobe Illustrator is desirable.

+ Proficiency with Safari, Chrome, and Firefox

+ Be proficient in use of Mac platform, and fluent in Microsoft-Word, -Excel, -PowerPoint

+ Be proficient in PubMed and PubMed Central literature searches.

+ Proficiency with the cBioPortal for Cancer Genomics, COSMIC, dbSNP, dbVar, ClinVar, ExAC, The Human Protein Atlas, Ensembl, NCBI Resources, DepMap, CCLE, GDSC, DGIdb, UniProtKB/Swiss-Prot and GTEx databases is highly desirable.

+ Proficiency in PolyPhen-2, SIFT, Mutation Assessor, PROVEAN, SNAP2, PANTHER-PSEP, and MutPred2 is desirable.

+ Proficiency in Clustal Omega, BLAST, and EMBOSS Seqret is desirable.

+ Proficiency in Partek Genomics Suite is desirable.

+ Proficiency in Ingenuity Pathway Analysis, g:Profiler, and/or DAVID is desirable.

+ Proficiency in EndNote is desirable


Field of Study


+ Biology


**Note: Vaccination against COVID-19 is a requirement for all job opportunities offered through Kelly Government Solutions, in accordance with the Executive Order on Ensuring Adequate COVID Safety Protocols for Federal Contractors.**


PLEASE APPLY ONLINE


When you apply, please know a Kelly Government Solutions Recruiter will review your resume and will contact you directly, if your skills and experience match the requirements of this position; a full job description can be made available at that time.


Due to the high volume of inquiries, we regret that we cannot accept phone calls. Even if you arent contacted for this position, you are still part of our talent network. All Kelly recruiters have access to your profile, which expands your opportunities even further.


As a specialty talent company, Kelly is committed to doing the right thing for our talent and clients. We stand up for equity, inclusion, fair treatment, and opportunity for all as these build the foundation of our Talent Promise. With a focus on safety, value, well-being, investment, and opportunity, Kellys Talent Promise confirms our commitment to those in search of a better way to work and live and to help discover whats next.


It is obvious things are different than they were just a few months ago. Kelly is at the forefront of ensuring the safety of our employees in this new working environment. Be well and stay strong.


**Why Kelly** **** **?**


Looking to connect with premier government agencies or the top 25 federal government contractors? Kelly Government offers a variety of full-time, project (SOW), seasonal, and surge support opportunities in science, engineering, technical, professional, and administrative support roles. Our experts will find you the opportunity that fits your schedule and interests and handle the details of transferring and processing all levels of security clearances. Its just another way we make the job search work for you.


**You should know:** Vaccination against COVID-19 is a requirement for all job opportunities offered through Kelly Government Solutions, in accordance with the Executive Order on Ensuring Adequate COVID Safety Protocols for Federal Contractors.


**About Kelly** ****


At Kelly, were always thinking about whats next and advising job seekers on new ways of working to reach their full potential. In fact, were a leading advocate for temporary/nontraditional workstyles, because we believe they allow flexibility and tremendous growth opportunities that enable a better way to work and live (plus, did we mention we provide a ton of benefits ?). Connecting great people with great companies is what we do best, and our employment opportunities span a wide variety of workstyles, skill levels, and industries around the world.


Kelly is an equal opportunity employer committed to employing a diverse workforce, including, but not limited to, minorities, females, individuals with disabilities, protected veterans, sexual orientation, gender identity Equal Employment Opportunity is The Law. (https://www.dol.gov/ofccp/regs/compliance/posters/ofccpost.htm)

Jobs Rated Reports for Biologist

Posted: 2021-10-22 Expires: 2021-12-09

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Molecular Biologist (Laboratory Management) - NIH - Bethesda, MD

Kelly Services
Bethesda, MD 20814

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