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Scientist: Cell Based Assay Fremont

Kelly Services


Location:
Fremont, CA
Date:
04/19/2018
2018-04-192018-05-19
Job Code:
US15TPSG_BH1435078
Kelly Services
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Job Details

Job Title Scientist: Cell Based Assay Fremont

Jobid US15TPSG_BH1435078

Location: Fremont, CA, 94536, USA

Description Life Scientist -

Scientist

Fremont

45/hr

Demonstrated successful experience in construct design, cloning and plasmid sequencing in a fast-paced, high-volume environment is essential. Knowledge and resourcefulness in modern methods for molecular biology for Work/Industry Experience Demonstrated successful experience in construct design, cloning and plasmid sequencing in a fast-paced, high-volume environment is essential. Knowledge and resourcefulness in modern methods for molecular biology for construct, virus and mammalian cell line generation.

Bachelor s degree or higher in molecular biology, cell biology, biochemistry or related discipline. Industry experience is strongly preferred. Work with various modes of gene delivery virus, lipid electroporation including retroviral or lentiviral vector systems is highly desirable. Experience with mammalian cell culture and cell-based assays while working under BSL1/2 is preferred. Flow cytometry/cell sorting, immunofluorescence and Western blotting skills are also desirable. Experience with inducible expression systems and CRISPR genome editing technologies is desirable. Knowledge of and interest in cell biology and signal transduction is a plus. Excellent organizational and communication verbal and written skills, with proven ability to multitask and work under demanding timelines. Must be comfortable and willing to work as both an individual contributor as well as an interactive team member.

Education degree and/or coursework /Certification , Minimum of 3-5 years as independent molecular biology contributor is required. Skills Duties /Software Essential Duties and Responsibilities: Design, generate and confirm the quality of expression plasmids required for all mammalian cell-based assay development activities at Eurofins DiscoverX. Regularly monitor and fulfill requests for new plasmid constructions and transfers of existing plasmids to cell lines. Actively maintain the Eurofins DiscoverX plasmid collection. Use bioinformatics tools including SnapGene and NCBI databases, etc. and strategic thinking for planning and executing molecular constructions. Generate and QC retrovirus and lentiviruses for mammalian gene delivery in vitro. Perform experiments to qualify new technologies to augment Eurofins DiscoverX capabilities. Communicate regularly with managers and other scientists to ensure orderly completion and transfer of plasmids from Molecular Biology to downstream assay development projects.

Maintain good documentation of laboratory work. Participate in departmental lab meetings. Create new cell lines for assays using retroviral/lentiviral transduction of proprietary constructs. Perform QC of cell lines and reagents for R D and Operations groups. Must follow and uphold departmental and company business procedures and rules. Other duties as required and assigned.

**Why Kelly** **®** **?**


With Kelly, you’ll have access to some of the world’s highest-regarded scientific organizations—providing you with opportunities to work on today’s most challenging, research-intensive, and relevantprojects. Our connections can lead you to innovative scientific pursuits you’ll be proud to helpadvance. We work with 95 of the Fortune 100™ companies, and found opportunities for more than 9,000scientific professionals last year.

You pursued a career in science to fuel your quest for knowledge and your desire to make the worlda better place. Let Kelly fuel your career—connect with us today.

**AboutKelly Services** **®**

As a workforce advocate for over 70 years, we are proud to directly employ nearly 500,000 people around the world and have a role in connecting thousands more with work through our global network of talent suppliers and partners. Revenue in 2017 was $5.4 billion. Visit kellyservices.com and connectwith us on Facebook , LinkedIn and Twitter .

Kelly Services is an equal opportunity employer including, but not limited to, Minorities, Females,Individuals with Disabilities, Protected Veterans, Sexual Orientation, Gender Identity and iscommitted to employing a diverse workforce. Equal Employment Opportunityis The Law.



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