28 days old

Scientist (Protein Purification/Production) - NIH - Bethesda, MD

Kelly Services
Bethesda, MD 20814
Kelly Government Solutions is a strategic supplier and business partner to the federal government and its key suppliers. Through our partnership with the National Institutes of Health, Kelly Government Solutions offers administrative, IT, engineering, scientific, and healthcare professionals the opportunity to work with one of the most prestigious research organizations in the world. We are currently seeking a **Scientist (Protein Purification/Production)** to work onsite with the National Institutes of Health in **Bethesda, MD.**

This is a long-term contract position which offers:

+ Competitive salary with comprehensive benefit package

+ Tremendous growth opportunity

+ Opportunity to work at NIH, the world's foremost medical research center

+ Learn more about what Kelly can do for you at

TASKS/SERVICES. The contractor shall:

+ Provide support for molecular/structural biology projects, as well as immunogen testing and analysis for an effective HIV vaccine and vaccines for other pathogens.

+ Prepare protein probes for B cell sorting and antibody isolation projects.

+ Perform B cell sorting using multicolor flow cytometry and single B cell sequencing to identify antibodies of interest.

+ Provide expression of viral glycoproteins, antibodies, immunogens and virus-like particles using mammalian expression systems; provide expression of immunogens in alternative expression systems.

+ Provide DNA vector manipulation including expression vector design, DNA/protein sequence analysis, DNA recombination, mutagenesis and plasmid preparation.

+ Provide biochemical analysis of proteins.

+ Maintain and generate stable cell lines.

+ Maintain tissue culture facility (maintenance of cell lines, cell culture mediums and supplies etc.).

REQUIREMENTS. The contractor must have:

+ Ph. D. in Biochemistry, Molecular Biology, Immunology or related discipline. Three (3) years of specialized experience plus a Masters degree is equivalent to a Ph. D. Five (5) years of specialized experience plus a Bachelors degree is equivalent to a Ph. D.

+ Minimum of two (2) years of experience in a related field.

+ Extensive experience with protein purification techniques

+ Affinity, Size Exclusion, Ion-exchange, Hydrophobic interaction chromatography etc.

+ Hands-on experience with biophysical characterization of proteins

+ Including but not limited to: size exclusion chromatography, dynamic light scattering, biolayer interferometry, surface plasmon resonance, differential scanning calorimetry

+ Experience with biotinylation and fluorescent labeling of protein probes for B cell sorting projects.

+ Experience with B cell isolation, FACS sorting, single B cell sequencing.

+ Experience working with mammalian cell culture for production of glycoproteins and antibodies

+ Basic knowledge of computer science, systems analysis, and systems programming.

+ Computer skills to include MS Word, Excel, and PowerPoint.

+ Experience with C, C++.

+ Sound knowledge of bioinformatics approaches to analyze.

+ A highly motivated Ph.D. person, able to follow explicit directions for protein production, but not required to carry out innovation.

+ Demonstrates prior experience working in a research laboratory environment; demonstrated ability to work as part of a team.


Note that the phone number for our NIH branch is not listed. Due to the high volume of inquiries, we regret that we cannot accept phone calls. All qualified candidates will be contacted.

**AboutKelly Services** ****

As a workforce advocate for over 70 years, we are proud to have a role in managing employment opportunities for more than one million workers around the globe. We employ 550,000 of these individuals directly with the remaining workers engaged through our talent supply chain network of supplier partners. Revenue in 2015 was $5.5 billion. Visit and connect with us on Facebook , LinkedIn and Twitter .

Kelly Services is an equal opportunity employer including, but not limited to, Minorities, Females, Individuals with Disabilities, Protected Veterans, Sexual Orientation, Gender Identity and is committed to employing a diverse workforce. Equal Employment Opportunity is The Law. at
Posted: 2019-12-30 Expires: 2020-01-30

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Scientist (Protein Purification/Production) - NIH - Bethesda, MD

Kelly Services
Bethesda, MD 20814

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