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24 days old

Scientist - Biochemistry and In Vitro Discovery

Kelly Services
San Diego, CA 92108
**Kelly Services is currently seeking a Scientist** **for a Direct Hire role in San Diego.**


**Job Title** : **Scientist - Biochemistry and In Vitro Discovery**


Pay: $80-90k depending on experience


We are seeking a Scientist-level candidates to implement general biochemical and molecular biological methods for the optimization and production of novel lasso therapeutics, as well as corresponding biosynthetic enzymes. Candidates must have relevant experience expressing and purifying proteins from a variety of organisms. A successful candidate will be expected to play a broad role in advancing our discovery platform. Specific areas of responsibility for this position will include, but are not limited to, propagation and manipulation a variety of bacterial organisms, isolation and characterization of lasso peptide pathway enzymes, and protein engineering to optimize pathway enzymes. Candidates will continuously interact with a multi-disciplinary team and make important contributions to our groundbreaking science in an exciting start-up environment where everyone makes a difference.


**Key Responsibilities** :


Culture and manipulate a variety of bacterial strains, including wild-type and engineered strains, with initial focus on gram-positive bacteria.


Design and build genetic constructs on self-replicating and integrating plasmids for heterologous overexpression of lasso peptide biosynthetic enzymes and pathways from various hosts.


Optimize protein expression and purify lasso peptide biosynthetic enzymes.


Develop biochemical assays to characterize enzymes and high throughput screening assays to rapidly monitor improved enzyme variants for enhanced production of lasso peptides of interest.


Develop methods for in vitro reconstitution of biosynthetic pathways and cell-free biosynthesis of lasso peptides for rapid production of analogs.


Acquire, evaluate, interpret, and present experimental results internally, at partner meetings, and at scientific conferences.


Execute safe laboratory procedures and always wear personal protection equipment in the labs.


Follow and help enforce all protocols associated with maintaining safe operations in the labs and offices during the COVID-19 pandemic.


**Qualifications and Skills** :


Ph.D. (or equivalent experience) in biochemistry, enzymology, molecular biology, microbiology, synthetic biology, or related disciplines (non-Ph.D. scientists with extensive relevant experience also will be considered). Industrial experience or postdoctoral work in these disciplines is desirable.


Skilled in overexpressing proteins from a range of bacterial strains (e.g., E. coli, Pseudomonas, Burkholderia, Bacillus, Streptomyces, etc.). Experience working with and genetically manipulating gram-positive organisms is desirable.


Proficient in recombinant DNA techniques and tools for constructing plasmids and vectors containing biosynthetic pathway genetic components e.g., DNA design, cloning, isolation, and sequencing).


Skilled in implementing and optimizing methods for protein production, isolation, and purification.


Expertise in developing biochemical assays for characterizing enzymes and enzyme complexes.


Proficient in bioinformatic analysis, BLAST search, sequence alignment, genomic database search and related methods and software.


Experience designing, refactoring, expressing, characterizing, and optimizing natural product biosynthetic pathways is desirable.


Strong publication history in peer-reviewed journals.


Ability to thrive in a fast-paced, dynamic, multi-disciplinary, team-driven work environment.


Must be detail-oriented, self-motivated, and able to work independently.


Excellent organization, strong interpersonal skills, excellent oral and written communication skills. Able to manage, organize, and critically evaluate data.


**Key Management and Leadership Competencies** :


Demonstrate leadership by example and take ownership and responsibility for advancing the companys mission every day.


Build strong relationships with other employees to enhance performance, enable achievement of results, and ensure delivery against goals and timelines.


Learn fast, grasp the essence and change course quickly when needed. Raise the bar and never be satisfied with the status quo.


Create a learning environment, open to suggestions for improvement.


Embrace the ideas of others, nurture a culture of trust, innovation, and accountability


**Why Kelly** **** **?**


Kelly Science & Clinical is your connection to premier scientific and clinical companies looking to hire talented people just like you. Every day, we match science professionals with dream jobs that fit their skills and interestsits the way we think job searching should be. Nearly 100 percent of our science recruiters have a professional background/education in science, so we know a thing or two about the science market and how to get you noticed.


**About Kelly** ****


At Kelly, were always thinking about whats next and advising job seekers on new ways of working to reach their full potential. In fact, were a leading advocate for temporary/nontraditional workstyles, because we believe they allow flexibility and tremendous growth opportunities that enable a better way to work and live (plus, did we mention we provide a ton of benefits ?). Connecting great people with great companies is what we do best, and our employment opportunities span a wide variety of workstyles, skill levels, and industries around the world.


Kelly is an equal opportunity employer committed to employing a diverse workforce, including, but not limited to, minorities, females, individuals with disabilities, protected veterans, sexual orientation, gender identity. Equal Employment Opportunity is The Law. (https://www.dol.gov/ofccp/regs/compliance/posters/ofccpost.htm)
Posted: 2021-10-01 Expires: 2021-11-18

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Scientist - Biochemistry and In Vitro Discovery

Kelly Services
San Diego, CA 92108

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