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1+ months

Scientist, Vector and Antibody Engineering

Kelly Services
Arcadia, CA 91007
**Kelly Science and Clinical** is the worlds most recognized brand in science and clinical staffing with more than 100 locations in North America, Europe, and the Pacific Rim. Our scientists contribute to the research and development of the next generation of small molecule drugs, biologics and cellular therapies.


**Overview**


We are seeking a Scientist, Antibody Engineering for one of our clients, a startup biotechnology company that specializes in manufacturing cell and gene therapies for cancer patients around the world. The incumbent will assist with genetic engineering of lentiviral vectors expressing antibodies and CAR and in vitro assays. The candidate will focus on the Discovery of Antibody based biologics and T/NK cell products as well as assist with planning and execution of experiments, in support of developing analytical methods for releasing and characterizing T cell therapy products. Candidates with strong expertise in phage display library construction and antibody engineering will be given preference. The successful candidate will demonstrate a strong sense of responsibility, open communication, desire to learn new skills, ability to meticulously perform instructed tasks and maintain lab records. S/he will perform bench work to support research projects in coordination with our scientific director. We are looking for enthusiastic individuals with a flexibility to adapt to startup environment.


**Responsibilities**


+ Use expertise in molecular biology to generate Chimeric Antigen Receptor (CAR) or antibody constructs.

+ Engineer lentiviral vectors with insertion or deletion of restriction sites, epitope tags or other genetic elements.

+ Generate viral vectors expressing scFv or CAR variants, site directed mutants, and fusion proteins using fusion PCR and Gibson or other fragment assembly methods.

+ Design and clone shRNA, gRNA for desired targets in CRISPR/Cas9 vectors.

+ Design strategies to knock-in CAR in T cells

+ Perform antibody phage display screens and validation of clones by ELISA and flow cytometry.

+ Express/Purify antibodies and recombinant proteins in bacteria or mammalian cells.

+ Develop biochemical, cell based and immunoassays to evaluate antibody binding affinities

+ Optimize qPCR/ddPCR assays to characterize viral and CAR-T products.

+ Construct phage display libraries

+ Perform restriction analysis, ligation, transformation, colony PCR screens and gel electrophoresis.

+ Develop generate stable cell lines with overexpression or knock out (CRISPR shRNA).

+ Prepare plasmids in small scale and large scale, maintain plasmid and bacterial glycerol stocks.

+ Maintain cell lines, prepare cell banks (adherent and suspension cell lines) as required.

+ Perform cytokine ELISAs and Western blotting.

+ Perform cell-based (proliferation, cytotoxicity, transcription factor reporter) assays in multi well formats, analyze and clearly document data.

+ Perform experiments with minimal supervision, think critically and demonstrate troubleshooting and problem-solving skills.

+ Self-motivated, pays attention to details, and willing to accept responsibilities outside of the initial job description

+ Develop precise SOPs complying with GLP environment and maintain excellent records that allow systematic tracking and protocol transfer.

+ Basic proficiency in various software (Excel, PowerPoint, Graph Pad Prism, Photoshop and Plasmid design/analysis software)


**Qualifications**


+ MS Degree preferably with at least 5+ years of laboratory experience or PhD with 2+ years of laboratory experience in the field of Immunology, cell biology, virology, or molecular biology.

+ **Experience in phage display library construction, antibody engineering, humanization or affinity maturation of antibodies are preferred.**

+ Strong technical skills in advanced molecular cloning and fragment assembly techniques are required

+ Strong experience in cell based and biochemical assays are required must be able to perform assays in 96 and 384 well formats with different formats without confusion.

+ Experience in working with multiple cell lines (both suspension and adherent) and setting up cell-based assays.

+ General lab experience in operating and maintaining basic lab equipment (centrifuge, microscope, PCR machines, nanodrop, DNA and protein electrophoresis systems).

+ Hands on experience with sterile microbiology and molecular biology techniques not limited to cloning, mutagenesis, restriction digestion, ligation, transformation, colony PCR, culturing bacterial cells, plasmid preparations.

+ Critical to have the ability to maintain accurate records and back track samples prepared without confusion.

+ Prior experience in optimizing and trouble-shooting qPCR and ddPCR

+ Communicate ideas and results clearly and effectively (both verbally and in writing) to the team members.

+ Must feel comfortable in a fast-paced small company environment with minimal direction and able to adjust workload based upon changing priorities

+ Ability to adapt to startup environment and make the best use of the existing resources to helping the team to move towards perfection

+ Familiarity with routine lab operations and safety procedures.


**You should know:** Your safety matters! Vaccination against COVID-19 may be a requirement for this job in compliance with current client and governmental policies. A Kelly recruiter will confirm and share more details with you during the interview process.


**Why Kelly** **** **?**


Kelly Science & Clinical is your connection to premier scientific and clinical companies looking to hire talented people just like you. Every day, we match science professionals with dream jobs that fit their skills and interestsits the way we think job searching should be. Nearly 100 percent of our science recruiters have a professional background/education in science, so we know a thing or two about the science market and how to get you noticed.


**About Kelly** ****


At Kelly, were always thinking about whats next and advising job seekers on new ways of working to reach their full potential. In fact, were a leading advocate for temporary/nontraditional workstyles, because we believe they allow flexibility and tremendous growth opportunities that enable a better way to work and live (plus, did we mention we provide a ton of benefits ?). Connecting great people with great companies is what we do best, and our employment opportunities span a wide variety of workstyles, skill levels, and industries around the world.


Kelly is an equal opportunity employer committed to employing a diverse workforce, including, but not limited to, minorities, females, individuals with disabilities, protected veterans, sexual orientation, gender identity. Equal Employment Opportunity is The Law. (https://www.dol.gov/ofccp/regs/compliance/posters/ofccpost.htm)
Posted: 2022-03-28 Expires: 2022-05-30

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Scientist, Vector and Antibody Engineering

Kelly Services
Arcadia, CA 91007

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