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9 days old

Senior Research Associate (Therapeutics) - IMMEDIATE NEED

Kelly Services
Arcadia, CA 91007
For over 25 years Kelly Science & Clinical has been connecting scientific professionals with meaningful work with industry leading employers. Today we are excited to be hiring a **_Senior_** **_Research Associate_** for an exciting therapeutics start-up in Arcaida, CA! The **_Sr. RA_** will assist with genetic engineering of lentiviral vectors expressing antibodies and CAR and in vitro characterization. You will be responsible for hands-on laboratory work, participating in the development of antibody-based biologics and CAR-T cell products. The Sr. RA will plan and execute experiments in support of developing analytical methods for releasing and characterizing T cell therapy products.


**Responsibilities:**


+ Process blood products for isolation of PBMC and immune cell subsets (T and NK) using magnetic bead-based technologies (MACs columns).

+ Generate CAR-T cells by lentiviral transduction and evaluate expression using flow cytometry, western blotting, or other binding assays.

+ Immunophenotype CAR-T products under different conditions to evaluate exhaustion and persistence using multicolor flow cytometry.

+ Perform T cell coculture functional assays to measure T cell activation and cytotoxicity, cytokine release (ELISA or intracellular staining by FACs), proliferation (CFSE)

+ Perform cell-based reporter assays, biochemical, and immunological assays.

+ Execute lentiviral/adenoviral production, concentration, and quantitation assays (Flow cytometry, ELISA, and qPCR).

+ Maintain mammalian cell lines (adherent and suspension) and generate cell banks

+ Generate stable cell lines using lentiviral, shRNA. CRISPR/Cas9 technologies.

+ Generate novel lentiviral antibody and CAR constructs using standard molecular biology techniques.

+ Engineer lentiviral vectors with insertion or deletion of restriction sites, epitope tags or other genetic elements.

+ Perform antibody phage display screens and validate hits by ELISA and flow cytometry.

+ Develop biochemical, cell-based and immunoassays to evaluate antibody binding

+ Perform experiments with minimal supervision, think critically and demonstrate troubleshooting and problem-solving skills.

+ Self-motivated, pays attention to details, and willing to accept responsibilities outside of the initial job description

+ Plan and execute lab work, analyze data, and keep lab records up to date and demonstrate ability to track them with clarity.


**Requirements:**


+ Bachelor's Degree, Master's Degree, or PhD in the field of Immunology, Virology, or related areas


+ Experience in working with multiple cell lines (both suspension and adherent) and setting up cell-based assays.

+ General lab experience in operating and maintaining basic lab equipment (centrifuge, microscope, PCR machines, nanodrop, DNA and protein electrophoresis systems).

+ Hands on experience with sterile microbiology and molecular biology techniques not limited to cloning, mutagenesis, restriction digestion, ligation, transformation, colony PCR, culturing bacterial cells, plasmid preparations

+ Experience in a variety of molecular, cellular, and biochemical techniques (cloning, transfections, transductions, western blotting, immunoprecipitation, qPCR gene manipulation using shRNA/CRISPR, luciferase reporter assays, flow cytometry)

+ Experience in viral vector production and transduction (lentiviral or retroviral or AAV)

+ Experience in multi-parameter flow cytometry and data analysis as well as different forms of biochemical and cell-based assays

+ Enthusiastic Researcher with a flexibility to adapt to startup environments


**Apply for Immediate Consideration**


**Schedule and interview with Athena** https://calendly.com/athp320/interview


**Text Regan at 303-601-8303 with any questions. Please be sure to include which job you are interested in.**


**You should know:** Your safety matters! Vaccination against COVID-19 may be a requirement for this job in compliance with current client and governmental policies. A Kelly recruiter will confirm and share more details with you during the interview process.


**Why Kelly** **** **Science & Clinical?**


Kelly Science & Clinical is your connection to premier scientific and clinical companies looking to hire industry experts just like you. Our team creates expert talent solutions to solve the worlds most critical challenges. Every day, we match science professionals with dream jobs that fit their skills, interests, and career goalsits the way we think job searching should be. Nearly 100 percent of our science recruiters have a professional background and education in science, so we know a thing or two about the science market and how to get your expertise noticed.


**About Kelly** ****


At Kelly, were always thinking about whats next and advising job seekers on new ways of working to reach their full potential. In fact, were a leading advocate for temporary/nontraditional workstyles, because we believe they allow flexibility and tremendous growth opportunities that enable a better way to work and live (plus, did we mention we provide a ton of benefits ?). Connecting great people with great companies is what we do best, and our employment opportunities span a wide variety of workstyles, skill levels, and industries around the world.


Kelly Services is proud to be an Equal Employment Opportunity and Affirmative Action employer. We welcome, value, and embrace diversity at all levels and are committed to building a team that is inclusive of a variety of backgrounds, communities, perspectives, and abilities. At Kelly, we believe that the more inclusive we are, the better services we can provide. Requests for accommodation related to our application process can be directed to Kellys Human Resource Knowledge Center.
Kelly complies with the requirements of Californias state and local Fair Chance laws. A conviction does not automatically bar individuals from employment.
Posted: 2022-09-23 Expires: 2022-10-24

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Senior Research Associate (Therapeutics) - IMMEDIATE NEED

Kelly Services
Arcadia, CA 91007

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